Articles, books and protocols

• These links will generate Entrez lists of P. vivax-related articles appearing in Pubmed in the
  • last week
  • last month
  • last year
  • last 5 years
  
  In addition, links to all P. vivax information available in the various NCBI databases are collected on the NCBI P. vivax Index page.


• Articles in PDF format from A Focus on Plasmodium vivax , a special publication of Trends in Parasitology that collected papers stemming from the first international P. vivax conference in 2002.


• Malaria and Plasmodium-related books currently listed on Amazon.com


• DPDx offers The Primate Malarias CD-ROM from the Division of Parasitic Diseases (DPD) at CDC. The CD-ROM is an electronic version of the now out-of-print book that was published in 1971, authored by G. Robert Coatney, William E. Collins, McWilson Warren, and Peter G. Contacos; permission to reproduce this book was granted by the authors. The book summarizes knowledge on different species of Plasmodium that develop in nonhuman primates. It has served as a foundation for subsequent investigations on these parasites, their primate hosts, and their mosquito vectors. DPD has made this electronic version available to the scientific community in hopes that it will stimulate and support continued interest in these parasites and their contribution to the understanding and control of malaria.


• Methods in Malaria Research (4th ed., Oct 2004) is a free compendium of protocols available from the Malaria Research and Reference Reagent Resource Center (MR4).
  The following links will load the entire ~300 page document in a new window. (To save the document without opening it, right-click on the link and select 'Save Link As'.)
  • MS Word
  • PDF

Reagents

• The Malaria Research and Reference Reagent Resource Center (MR4):
  Type the word 'vivax' in the 'Search For:' box on this page and choose 'Search All' to generate a list of all MR4 P. vivax reagents (antibodies, cell lines, parasites, proteins/peptides, genomic DNAs, libraries, and plasmid clones/vectors).

MR4 whole genome-scale reagents used in published work include:
• 6,000 genome survey sequence (GSS) clones from a mung bean nuclease library of P. vivax Salvador I, available as glycerol stocks: (MRA-292) or miniprep DNA: (MRA-293). [PMID: 11738710]
• 4,000 genome survey sequence (GSS) clones from a mung bean nuclease library of P. vivax Belem, available as glycerol stocks: (MRA-294) or miniprep DNA: (MRA-295). [PMID: 11738710]
• 800 cDNA clones from a cDNA library from a Brazilian patient infected with P. vivax, available as glycerol stocks: (MRA-509). [PMID: 12914668]
• ~20,000 cDNA clones from a cDNA library from a Thai patient infected with P. vivax, available as glycerol stocks: (MRA-760). [PMID: 16085323]
• ~3,800 genomic DNA clones from various DNA libraries of Salvador I used during the P. vivax genome sequencing project and representing a tiling path of clones across each chromosome, available as glycerol stocks: MRA-840 [PMID: 12763429]


• Pathogen Functional Genomics Resource Center (PFGRC):
• long-oligo microarray of P. vivax Salvador I.

Isolates

In The Contribution of Genetics to the Study of Parasitic Protozoa (D. Walliker, 1983), the following terms are defined:

isolate

a sample of parasites collected on a single occasion from a natural host, and subsequently maintained in the laboratory. An isolate is not necessarily genetically homogenous, and may even contain representatives of more than one species

line

a collection of parasites which have undergone a particular passage or treatment. The parasites in a line usually have certain characteristics in common, but are not always genetically identical

clone

a group of genetically identical organisms derived from a single cell by asexual reproduction

Below is a table of some of the isolates of P. vivax that have been adapted to New World monkeys. Many of these are used as 'standard strains' for the study of vivax malaria, e.g, Salvador I, which was used for the first whole genome shotgun sequencing project.